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| Paiva, Vera Lisa Generosa da Silva; Souza, Alex Junior Souza de; Naranjo, Luis Fabian Núñez; Parra, Silvana Hipatia Santander; Ferreira, Claudete Serrano Astolfi; Ferreira, Antonio José Piantino; Massironi, Silvia Maria Gomes; Mori, Claudia Madalena Cabrera; Sá, Lilian Rose Marques de. |
| Os ratos (Rattus norvegicus) são os roedores mais usados em pesquisas biomédicas. Para que os resultados obtidos nos experimentos sejam corretos, precisos e reproduzíveis é imprescindível que a condição sanitária dos animais seja objeto de cuidados especiais. O Mycoplasma pulmonis, causador da micoplasmose respiratória murina (MRM), é um dos agentes potencialmente causadores de interferências nos resultados de investigações experimentais. O presente trabalho foi delineado para efetuar a caracterização histopatológica dos pulmões de ratos Wistar nas várias faixas etárias, assim como a detecção de Mycoplasma spp. com o emprego das técnicas de ELISA e moleculares |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ratos Wistar; Pulmão; Mycoplasma pulmonis. |
| Ano: 2016 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31079 |
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| Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.. |
| This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015 |
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| Delgado,Márcio Oliveira; Timenetsky,Jorge. |
| Mycoplasma pulmonis have been isolated in about 10(5) CFU/mL from tracheal aspirates of rats from conventional animal facilities in São Paulo. The mycoplasma transmission by aerosol may happen from an infected rat to a healthy one at distances up to 120 cm. This condition also favors the technicians contamination. As this infection is unknown in humans, in this study the immunoblot profiles to M. pulmonis of sera from rats were compared to those presented by animal facility technicians. About 32 proteins from 11 to 230 kDa (kilodaltons) were recognized by the sera from rats naturally infected with M. pulmonis. Sera from technicians responsible for the cleaning and sanitation of cages of infected animals for more than seven years recognized about 10... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma pulmonis; Human sera; Animal facilities. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400009 |
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